Ali ghavampour; Mohammad Ali Salari Aliabadi; Hossein Zolgharneen; bita archangi
Abstract
Resource genetics idendtification is one of the most efficient strategies for fisheries management regarding commercially important marine resources.One of the valuable species of shrimp in the Persian Gulf is the banana shrimp (Fenneropenaeus merguiensis), which accounts for about 60% of total annual ...
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Resource genetics idendtification is one of the most efficient strategies for fisheries management regarding commercially important marine resources.One of the valuable species of shrimp in the Persian Gulf is the banana shrimp (Fenneropenaeus merguiensis), which accounts for about 60% of total annual shrimp catch in Hormozgan province.Identifying the genetic structure of the country's marine reserves is an effective way of efficient fisheries management. In this study, the amount of genetic diversity in the populations of this species was studied using microsatellite markers by 10 primers in four populations at Jask, Kolahi, Bandar Abbas power plant and Tula catch area and their genetic characteristics were compared with a 1 gram shrimp batch as a sample from stock enhancement project which was being stocked near kolahi area. Results of Genetic Distance Matrix, Heterozygosity, Assignment Test and Hardy-Weinberg Equilibrium at the Locations Indicated Average Population Distance (over 0.05 ) and Possible Effects of nursery ground and Regional Hydrodynamic Flows.
Rahim Abdi; Abdullah Reesi; Ahmad Savari; Mohammad Ali Salari Aliabadi; Gholam Reza Eskandari
Abstract
For this research, the required Acanthopagrus latus fish were selected by the expected catch method and finally the same size samples with an average weight of 150 g were selected. Then, five pieces of fish in 300 liter aquariums containing water with different salinities including Khor Musa with a mean ...
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For this research, the required Acanthopagrus latus fish were selected by the expected catch method and finally the same size samples with an average weight of 150 g were selected. Then, five pieces of fish in 300 liter aquariums containing water with different salinities including Khor Musa with a mean salinity of 42.7 ± 0.34 ppt and Arvand with a mean salinity of 14.4 ± 0.29 ppt were exposed to 0.1 LC5096 obtained from the lethal toxicity of silver nanoparticles and silver nitrate. For each environment and control treatment, after two weeks of adaptation, they were placed with three replications. The sampling was performed at 1, 6, 12 and 24 hours from the caudal stalk vein with 2 cc syringes and 5 millimeter samples of liver at the end of the experiment. To separate the serum, blood samples without anticoagulant were centrifuged for 15 minutes at 3000 rpm. Finally, the activity of ALP, AST and ALT enzymes was measured and evaluated by an autoanalyzer device using diagnostic kits. Also, to prepare microscopic micrographs, paraffin sections of liver tissue were stained with hematoxylin-eosin. Results showed that two different forms of silver, including silver nitrate and silver nanoparticles, had different effects on the health and liver function of the Acanthopagrus latus which was evident in the treatment of silver nitrate. This action has been seen as a change in the activity of serum enzymes especially the increase in more times, necrosis, hyperemia, hemorrhage, biliary regurgitation and vacuolar degeneration especially at low salinities.
علوم زیستی دریا
hedayat yavarmoghadam; h zolgharnein; Mohammad Ali Salari Aliabadi; s keyvan; Mohamad Modarresi
Abstract
In this present study genetic diversity of cuttlefish (Sepia pharaonis) populations were investigated using microsatellite markers. Total 51 samples were collected from Bandarabass and Bushehr regions. Tissue sample of arm tips (tentacle) were preserved in 96% ethanol alcohol until using in biotechnology ...
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In this present study genetic diversity of cuttlefish (Sepia pharaonis) populations were investigated using microsatellite markers. Total 51 samples were collected from Bandarabass and Bushehr regions. Tissue sample of arm tips (tentacle) were preserved in 96% ethanol alcohol until using in biotechnology laboratory of Khorramshahr University of Marine Science and Technology. Genomic DNA was extracted with CTAB method. The quality and quantity of extracted DNA was assessed by 1% agarose gel electrophoresis and spectrophotometry, respectively. Polymerase chain reaction conducted with 6 pairs of microsatellite primers. PCR products were electrophoresised on 8% polyacrylamide gel and stained with silver nitrate. These primers were shown 4 pairs of polymorph and 2 pairs of monomorp. Allele Sizes were measured in populations then genetic parameter were calculated using Arlequin and Gen Alex Programs and phylogenetic relationship was determinated and drawn using TFPGA Program. Result obtained showed genetic distance and resemblance distance is 0.282 and 0.754, respectively and genetic differentiation was present 0.031. The findings of present study showed low levels of genetic differentiation but significant between of populations and S. pharaonis were likely two different population. These findings provide useful information for conservation and management of this species in the Persian Gulf.
m r; h z; v y; m s; b a
Volume 12, Issue 4 , September 2014, , Pages 40-50
Amir Jaferian; Hosein Zolgharnein; Mehdi Mohammadi; Mohammad Ali Salari Ali Abadi; Seyyed Javad Hoseini
Volume 12, Issue 1 , April 2013, , Pages 29-37
Abstract
Fourfinger Threadfin in shallow coastal waters with sandy or muddy bottom and into the lives of the rivers. The fish in the coastal areas of tropical and subtropical areas of the environment. The family of 7 genera and 35 species have been identified in the warm waters. Persian Gulf fish are apart from ...
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Fourfinger Threadfin in shallow coastal waters with sandy or muddy bottom and into the lives of the rivers. The fish in the coastal areas of tropical and subtropical areas of the environment. The family of 7 genera and 35 species have been identified in the warm waters. Persian Gulf fish are apart from the economic elite. Sampled using boats equipped with fishing hooks gill net and sometimes in the Persian Gulf was. And was amplified using the number six position. The highest number of alleles observed in the samples and place Eletet2 Bushehr (5 alleles) and the lowest in the province is the place Eletet16.0 and the samples (one allele). Ho Eletet16.0 samples collected in the range of the lowest position of the province and the province is the highest position Eletet16.0. Shannon index Eletet2 highest position in the province) 1.494 (and its lowest position in the province Eletet17 (0.637) is. AMOVA tests based on risk level 0.01 differences between samples (%76), the difference between the groups (regions) (%0), differences between regions (%24) was calculated. similar genetic criteria Nei, (1972) The province of Khuzestan and Bushehr specimens (0.486) and genetic distance between samples of Khuzestan and Bushehr (0.615) exists. In the present study, heterozygosity at the highest position in the province of Bushehr is Eletet2. The highest observed heterozygosity (Ho), 0.923 is the position Eletet16.0 and Bushehr specimens. The amount of heterozygosity observed in this study is more indicative of the amount expected heterozygosity genetic variability due to fish migration in the near and far and free access to water (high gene flow) is. The results suggest that it is probably a fish population in the areas of Fourfinger Threadfin conventional fisheries management perspective there is any, is the same.